Fugene hd transfection reagent manual

FuGENE ® HD Transfection Reagent 8µl 7µl 6µl 5µl 4µl 3µl 2. ®Incubate the FuGENE HD Transfection Reagent/DNA mixture for 0 15 minutes at room temperature. 3. Add 2 10µl of the FuGENE ® HD Transfection Reagent/DNA mixture per well to a well plate containing µl of cells in growth medium. Mix by pipetting or using a plate shaker. Formulation and Packaging: FuGENE® HD Transfection Reagent is a proprietary mixture of lipids and other components in 80% ethanol, sterile filtered and supplied in sterile glass vials. It does not contain any ingredients of human or animal origin. Special Handling: Allow FuGENE® HD Transfection Reagent to reach room temperature, and mix briefly by. FuGENE ® HD is a next-generation, % synthetic, multi-component transfection reagent designed for the delivery of DNA into more challenging eukaryotic and insect cell lines. FuGENE® HD interacts with nucleic acids the cell’s membrane to provide efficient and safe entry into the cell. This mechanism allows users to overcome barriers in difficult-to-transfect lines such as primary cells, stem cells, and .

Transfection Reagent. Mirus Bio™ Trans IT™-LT1 Transfection Reagent. High-efficiency plasmid DNA delivery in many mammalian cell types, including primary cells. The Trans IT™-LT1 Transfection Reagent is broad spectrum, low toxicity, and serum compatible, and is suitable for both transient and stable transfection. Pricing Availability. FuGENE® HD Transfection Reagent. トランスフェクション困難な細胞株を含む広範な細胞腫に、より優れたパフォーマンス. E, E ViaFect™ Transfection Reagent. iPS細胞など様々な細胞株に使える、高効率・低毒性なトランスフェクション試薬. E, E TransFast. many commercially available transfection reagents. FuGENE® HD Transfection Reagent can be used in transfection protocols in the presence of serum (up to %), allowing transfection of cell types that require continuous exposure to serum, such as primary cell cultures. 3.E. Antibiotics Antibiotics can be used during the culture of cell lines.

FuGENE® HD Transfection Reagent(a) is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture. Incubate the FuGENE® 6 Transfection Reagent/DNA mixture for 15 minutes at room temperature. Note: Incubations longer than 45 minutes may adversely aff ect transfections. 3. Add 2–10µl of the FuGENE® 6 Transfection Reagent/DNA mixture to each well of a well plate containing µl of cells in growth medium. Formulation and Packaging: FuGENE® HD Transfection Reagent is a proprietary mixture of lipids and other components in 80% ethanol, sterile filtered and supplied in sterile glass vials. It does not contain any ingredients of human or animal origin. Special Handling: Allow FuGENE® HD Transfection Reagent to reach room temperature, and mix briefly by.